FastQCFastQC Report
Sat 31 Dec 2022
SRR1954641.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954641.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320956
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32131.0010717980034647No Hit
GAATCTATCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC19520.6081830531287777No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCG8490.26452223980857187No Hit
GAACTGACTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT6100.19005720410274304No Hit
GAATGAACTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTC4990.1554730243397849No Hit
CGCTGTATCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG4780.14893007141165768TruSeq Adapter, Index 23 (95% over 23bp)
GAATGCAGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT4700.14643751791522827No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGC4560.14207554929647678No Hit
CGTTTCAGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT4370.1361557347424569No Hit
CCTGTCACTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC3920.12213512132504144TruSeq Adapter, Index 23 (95% over 24bp)
GCTGTCACTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC3780.11777315270628996TruSeq Adapter, Index 23 (95% over 24bp)
GAATGAATGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT3350.10437567766298185No Hit
CGTTTTATGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT3290.10250626254065978No Hit
CGTTCTATCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC3280.1021946933536061TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3270.10188312416655242No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)