FastQCFastQC Report
Sat 31 Dec 2022
SRR1954637.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954637.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124302
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.9243616353719168No Hit
GAATCTATCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC5120.41190004987852163No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG4290.3451271902302457No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC3980.3201879293977571No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3340.26870042316294185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2690.21640842464320767No Hit
CTGTCTATTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT2170.17457482582742032Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCACTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC1890.15204904184968868Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCACTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC1510.12147833502276714Illumina Single End Adapter 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC1440.11584688902833422No Hit
GAATGAATGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT1410.11343341217357726No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG1360.10941095074898231No Hit
GAATGAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC1330.10699747389422536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)