FastQCFastQC Report
Sat 31 Dec 2022
SRR1954635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177639
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.502141984586718No Hit
GAATCTATCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC3520.1981546845005883TruSeq Adapter, Index 23 (95% over 22bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG3050.17169653060420292No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGC2810.15818598393370825No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.1356684061495505No Hit
CTGTCTATTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT2020.11371376780999669TruSeq Adapter, Index 23 (96% over 26bp)
GCTGTCACTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC1910.1075214339193533Illumina PCR Primer Index 7 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.1075214339193533No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)