FastQCFastQC Report
Sat 31 Dec 2022
SRR1954632.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954632.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425435
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52401.2316805152373453No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT13540.31826248428079496No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC11290.2653754392562906No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC10030.2357586940425682No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9010.21178323363145957No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG7290.17135402587939402No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7170.16853338347808713No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC6800.1598364027407242No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC6530.1534899573377837No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC6050.1422073877325561No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGC5830.13703620999682678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)