FastQCFastQC Report
Sat 31 Dec 2022
SRR1954630.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954630.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300531
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.8864310170997335No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC14240.47382799112238TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT13220.4398880647919849TruSeq Adapter, Index 27 (100% over 25bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC11370.37833035527117004TruSeq Adapter, Index 27 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC6250.20796523486761764TruSeq Adapter, Index 27 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC5260.175023541664587TruSeq Adapter, Index 27 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC5110.1700323760277642TruSeq Adapter, Index 27 (100% over 24bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.1450765478436501No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG3830.12744109592687608No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGC3100.10315075649433836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.10148703461539742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)