FastQCFastQC Report
Sat 31 Dec 2022
SRR1954629.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954629.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111790
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7570.6771625368995439No Hit
CTGTCTATTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT2430.2173718579479381TruSeq Adapter, Index 27 (100% over 25bp)
GCTGTCACTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC2100.18785222291797118TruSeq Adapter, Index 27 (96% over 25bp)
CCTGTCACTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC2090.18695768852312372TruSeq Adapter, Index 27 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1890.16906700062617408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.12165667769925755No Hit
TCTGTCACTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC1280.11450040254047768TruSeq Adapter, Index 27 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)