FastQCFastQC Report
Sat 31 Dec 2022
SRR1954628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164381
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16370.9958571854411399No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC9180.5584587026481163No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG8670.5274332191676654No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGC7210.4386151684197079No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC5240.31877163419129945No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT4840.2944379216576125Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC4080.24820386784360723No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3260.19831975714954894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.1721610161758354No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTC2240.1362687901886471No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCT2090.1271436479885145No Hit
GAACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT2040.12410193392180362No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCC2030.12349359110846143No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC1900.11558513453501318No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTACTCGGT1750.10645999233488054No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)