FastQCFastQC Report
Sat 31 Dec 2022
SRR1954627.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954627.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68510
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.683111954459203No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG2210.3225806451612903No Hit
GAATCTATCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC1890.27587213545467815No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGC1570.22916362574806598No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.19121296161144358No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.1882936797547803No Hit
CTGTCTATTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT1190.17369727047146402Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCACTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC1160.1693183476864691No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC860.12552911983652024No Hit
CCTGTCACTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC740.10801342869654065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)