FastQCFastQC Report
Sat 31 Dec 2022
SRR1954618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291228
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30831.058620737017045No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC7950.27298199348963703No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG7880.27057837845262134No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC6580.22593981347947312No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC5750.19743980661200158No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT5400.18542173142692325Illumina Single End Adapter 1 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5150.17683739200901014No Hit
GGTAATGGGAACATCATGAACAAGATATTAAATTGTACCTAAGGATTTGTA4380.15039762660183775No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4190.14387352864422379No Hit
CCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC3880.13322894776601152No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCC3560.12224099331108274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)