FastQCFastQC Report
Sat 31 Dec 2022
SRR1954616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571287
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71131.2450834694295512No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.21582847150381507No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC9480.1659411119104758No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCC9270.1622652012035982No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT8790.1538631195878779Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC7040.12323053036389765No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCG6880.12042983649199089No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC6720.11762914262008412No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6400.11202775487627059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)