FastQCFastQC Report
Sat 31 Dec 2022
SRR1954613.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954613.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135170
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.7250129466597618No Hit
GAATCTATCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC8470.6266183324702227No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG6780.501590589627876No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC5630.4165125397647407No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.22934083006584302No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC2750.2034475105422801No Hit
GAATGAACTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTC2330.17237552711400458No Hit
GAACTGACTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCT2200.16275800843382407No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG2100.15535991714137753No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC1900.14056373455648444No Hit
GAATGAATGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT1890.1398239254272398No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1890.1398239254272398No Hit
GCTGTCACTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC1870.13834430716875046TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTATTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT1460.10801213286971961TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph