FastQCFastQC Report
Sat 31 Dec 2022
SRR1954612.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954612.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768067
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63700.8293547307721851No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC39790.5180537635388579No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT27540.35856246915959156Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC23400.3046609215081497No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC22320.29059964820777356No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG19820.25805040445690286No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGC13890.18084359827983756No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC12770.1662615370794475No Hit
GAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT11850.15428341537912707No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.13931076325372657No Hit
ACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC9320.12134358070324594No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTT8750.11392235312804742No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTC7820.10181403445272354No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)