FastQCFastQC Report
Sat 31 Dec 2022
SRR1954608.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954608.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202310
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20010.9890761702337997No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTC5950.29410310909001036No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG5190.2565369976768326No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGC4670.23083386881518464No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.17102466511788839No Hit
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCT3440.17003608323859423Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCC3140.15520735504918193No Hit
GCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC2880.14235579061835796Illumina PCR Primer Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGC2520.12456131679106322Illumina PCR Primer Index 8 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.10775542484306262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)