FastQCFastQC Report
Sat 31 Dec 2022
SRR1954606.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954606.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504282
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50821.0077694623246516No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC27310.5415620624967776No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC25800.5116184991730818TruSeq Adapter, Index 16 (100% over 23bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT23550.4670006068033362TruSeq Adapter, Index 16 (96% over 25bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG23020.4564906143784629No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC20040.3973966947065332TruSeq Adapter, Index 16 (100% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC16880.334733343645024No Hit
TCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC11070.2195200304591479TruSeq Adapter, Index 16 (100% over 23bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8620.17093610321209166No Hit
ACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC8440.167366671822512TruSeq Adapter, Index 16 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT7200.1427772555831856TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTC6430.12750802130553937No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6150.12195557247730436No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCT6060.12017085678251455No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC5610.11124727830856544No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACCCTCT5340.10589313122419598No Hit
AGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG5050.10014238065209546TruSeq Adapter, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)