FastQCFastQC Report
Sat 31 Dec 2022
SRR1954605.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954605.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187749
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.706262083952511No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.19973475224901333No Hit
GAATCTATCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC3540.18854960612306856TruSeq Adapter, Index 16 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG3080.16404880984718961No Hit
GCTGTCACTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC3010.16032042780520803TruSeq Adapter, Index 16 (100% over 23bp)
CTGTCTATTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT2950.1571246717692238TruSeq Adapter, Index 16 (96% over 25bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC2670.1422111436012975No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2400.12783024143936852No Hit
CCTGTCACTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC2310.1230366073853922TruSeq Adapter, Index 16 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)