FastQCFastQC Report
Sat 31 Dec 2022
SRR1954604.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954604.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310763
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24960.8031844202816938No Hit
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC15320.4929801810382832No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG12270.3948346489125153No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC10330.33240765470792855No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC6530.2101279753381194TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT6440.20723187766883444TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC5010.16121610359019573TruSeq Adapter, Index 13 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.13708195634615447No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT4140.13322049278710785No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC4140.13322049278710785No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3480.11198244321235153No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC3470.11166065458243098No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT3280.10554667061394053No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT3280.10554667061394053No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)