FastQCFastQC Report
Sat 31 Dec 2022
SRR1954603.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954603.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126208
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6990.5538476166328601No Hit
GAATCTATCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC3090.24483392494929007No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG2290.1814465010141988No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC2210.17510775862068967No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.1449987322515213No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1830.1449987322515213No Hit
CTGTCTATTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT1350.10696627789046652TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCACTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC1270.1006275354969574TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)