FastQCFastQC Report
Sat 31 Dec 2022
SRR1954602.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954602.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513272
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95571.861975716579124No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC79941.5574588132608052No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG59921.1674122102900606No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC52751.0277201951401986No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC20630.4019311398244985No Hit
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT18560.36160164591094TruSeq Adapter, Index 15 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT17660.3440670833398276No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.3146479839149613No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTAT15840.3086083012515781No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC15380.29964619149300953TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA12250.23866487944014092No Hit
GAATATGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC11760.22911828426253528No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG9520.18547670630776664No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT9470.18450256394270484TruSeq Adapter, Index 15 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC8660.1687214576287037TruSeq Adapter, Index 21 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCC8560.1667731728985801No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC7900.1539144936797643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7510.1463161832322823No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT6760.1317040477563553No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)