FastQCFastQC Report
Sat 31 Dec 2022
SRR1954601.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954601.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205059
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25561.2464705279943822No Hit
GAATCTATCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC17850.870481178587626No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG14540.7090642205414052No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC12700.6193339477906359No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.3696497105711039No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC5500.26821548920066907No Hit
GAATGAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC5080.24773357911625438No Hit
GAATGAATGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT4400.21457239136053527No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG4210.2053067653699667No Hit
GAACTGACTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT4110.20043012011177272TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC3690.17994821002735797No Hit
GCTGTCACTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC3420.16678126783023423TruSeq Adapter, Index 21 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3270.15946629994294326No Hit
GAATCTATCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTT2810.13703373175525094No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.12581744766140476No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2520.12289146050648839No Hit
GAATGCAGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT2100.10240955042207364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)