FastQCFastQC Report
Sat 31 Dec 2022
SRR1954600.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954600.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287478
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC58012.017893543158085No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG55941.945888033171234No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC55081.915972700519692TruSeq Adapter, Index 22 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41131.4307181766952601No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACGTGCT16130.5610864135690383No Hit
GAACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT15920.553781506758778No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTC13650.4748189426669171No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT13610.4734275318459152No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTA13130.4567306019938917No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC12690.44142508296287025TruSeq Adapter, Index 22 (95% over 24bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG9220.3207201942409506No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC8600.2991533265154203No Hit
GAATATGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC8290.28836989265265517TruSeq Adapter, Index 22 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7570.26332449787462No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT6700.23306131251782744Illumina Single End Adapter 2 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5920.20592880150828932No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGT5380.18714475542476294No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCT5020.17462205803574535No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC5020.17462205803574535TruSeq Adapter, Index 22 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCTGTCTC4940.17183923639374143No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4320.15027236866821114No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT4290.14922881055245968No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC4110.14296746185795087No Hit
GAATAATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGACGTGCT3260.11339998191165933No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATG3240.11270427650115836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)