FastQCFastQC Report
Sat 31 Dec 2022
SRR1954599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109586
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC12831.170769988867191No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG11491.048491595641779No Hit
GAATCTATCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC11171.0192907853192925No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11021.0056029054806273No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC3810.34767214790210427No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG3430.31299618564415166No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.3066085083861077No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC3210.2929206285474422TruSeq Adapter, Index 22 (95% over 21bp)
GAACTGACTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT2890.2637198182249557No Hit
GAATGAACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTC2850.260069716934645No Hit
GCTGTCACTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC2650.2418192104830909TruSeq Adapter, Index 22 (95% over 24bp)
GAATGAATGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT2580.23543153322504698No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2410.21991860274122604No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2150.19619294435420584No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCT1800.16425455806398628No Hit
GAATGAAACGGCGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTA1700.15512930483820928No Hit
GAATCTATCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCTGTCTC1630.14874162758016535No Hit
GAATCTATCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTT1560.14235395032212145No Hit
CTGTCTATTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT1270.1158907159673681Illumina Single End Adapter 2 (95% over 23bp)
CCTGTCACTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC1230.1122406146770573TruSeq Adapter, Index 22 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)