FastQCFastQC Report
Sat 31 Dec 2022
SRR1954598.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954598.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192794
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25131.303463800740687No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG23691.228772679647707No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC20741.0757596190752825No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC19431.0078114464143075No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATAAGTGT6920.3589323319190431No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC6830.3542641368507319TruSeq Adapter, Index 15 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT5830.3023953027583846No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC5710.29617104266730293No Hit
GAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT4900.2541572870525016No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.246895650279573No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT4640.24067139018849135TruSeq Adapter, Index 21 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG4200.21784910318785855No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC4090.21214353143770032TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTA3490.18102223098229198No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC3370.1747979708912103No Hit
GAATATGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC3220.1670176457773582No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.1561251906179653No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC2840.14730748882226624No Hit
GAATGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT2650.13745241034472028No Hit
AGTTGAAATTTTCTTATTTTTTTAAAAATTCTTTTCAAAAGGAATTATTTA2140.11099930495762317No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)