FastQCFastQC Report
Sat 31 Dec 2022
SRR1954597.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954597.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78776
Sequences flagged as poor quality0
Sequence length51
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.8682847567787142No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG5590.7096069868995634No Hit
GAATCTATCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC5130.6512135675840357No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC4470.567431705087844No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.26150096476084084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.20818523408144612No Hit
GAATGAATGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT1630.2069158119224129No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC1630.2069158119224129No Hit
GCTGTCACTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC1560.19802985680918045TruSeq Adapter, Index 15 (95% over 22bp)
GAATGAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC1500.19041332385498122No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG1460.18533563521884838No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC1380.1751802579465827No Hit
AGTTGAAATTTTCTTATTTTTTTAAAAATTCTTTTCAAAAGGAATTATTTA1240.1574083477201178No Hit
GAACTGACTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT1140.1447141261297857No Hit
CTGTCTATTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT1000.1269422159033208TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCACTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC840.10663146135878948TruSeq Adapter, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)