FastQCFastQC Report
Sat 31 Dec 2022
SRR1954596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278397
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.8164599474850662No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTC11470.41200156610883015No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG9800.35201528752105804No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC8660.3110665704012615No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC5190.1864244226769685No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT5140.18462842631206514No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC4290.15409648810870807No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.14942689755995933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3780.1357773251866938No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC3440.123564549905351No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT3040.10919657898612414No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCAAGCGT2930.10524538698333676No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTT2920.10488618771035607No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)