FastQCFastQC Report
Thu 19 Jan 2023
SRR1954593.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954593.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101003
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.9554171658267576No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.28018969733572274No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.16732176271991922No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.10197716899498036No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTGC207.0027326E-445.00000420
TGTCACT302.1458236E-645.0000043
CTGTCAC302.1458236E-645.0000042
GGAGCTT207.0027326E-445.0000048
GGATGCC207.0027326E-445.0000048
CGGTTTA207.0027326E-445.0000041
AAGGTAT207.0027326E-445.0000046
CGATTGG207.0027326E-445.00000410
TCTACTA207.0027326E-445.00000424
AGGTTCA207.0027326E-445.0000047
GTCACTT302.1458236E-645.0000044
GGAGTGT207.0027326E-445.0000048
GCAGGAT207.0027326E-445.0000045
GCAGGAC207.0027326E-445.0000045
CACACCC207.0027326E-445.00000429
GGCGATA406.708433E-945.0000048
GTAAGGC406.708433E-945.0000044
TACAGGA207.0027326E-445.0000044
CCTATCT207.0027326E-445.0000044
CCAGTCT207.0027326E-445.0000045