FastQCFastQC Report
Sat 31 Dec 2022
SRR1954590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233774
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24801.0608536449733503No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC23761.0163662340551132No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG22770.9740176409694833No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC13400.5732031791388265No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC7390.3161172756593975TruSeq Adapter, Index 23 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT6380.2729131554407248No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT6180.2643578841102946No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTC6070.25965248487855797No Hit
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT4940.21131520186162706TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC4820.20618203906336888TruSeq Adapter, Index 23 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTTCTACT4740.2027599305311968No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTA4480.19163807780163747No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.18137175220512117No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG3780.1616946281451316No Hit
GAATATGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC3480.14886172114948626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.1334622327547118No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC2930.1253347249908031No Hit
TCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC2410.10309101953168444TruSeq Adapter, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)