FastQCFastQC Report
Sat 31 Dec 2022
SRR1954589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94566
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7100.7507983841972803No Hit
GAATCTATCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC6910.7307065964511558No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG5310.5615125943785293No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC3160.3341581540934374No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.2336992153628154No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC1970.2083201150519214No Hit
GCTGTCACTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC1930.20409026500010577TruSeq Adapter, Index 23 (95% over 23bp)
GAATGAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTC1670.17659623966330393No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG1580.1670790770467187No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.16179176448194912No Hit
GAACTGACTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT1400.14804475181354823No Hit
GAATGAATGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT1330.1406425142228708No Hit
CTGTCTATTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT1270.1342977391451473TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCACTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC990.10468878878243766TruSeq Adapter, Index 23 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)