FastQCFastQC Report
Sat 31 Dec 2022
SRR1954586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543143
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35760.658390147714322No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC22000.40504986716205493No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG19300.35533920164671184No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC13970.25720666564790484No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC12850.23658594513783662TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT10270.18908464253428656TruSeq Adapter, Index 8 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCC7320.13477113761937465No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC7310.1345870240433919TruSeq Adapter, Index 13 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT6880.12667014027613355TruSeq Adapter, Index 8 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6530.12022616511673723No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.10328771612632401No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)