FastQCFastQC Report
Sat 31 Dec 2022
SRR1954585.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954585.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214310
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.4740796043115113No Hit
GAATCTATCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC4930.23004059539918809No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG3820.17824646540058792No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC3100.14465027296906352No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.13858429378003828No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2950.1376510662124959No Hit
GCTGTCACTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC2300.10732117026736969TruSeq Adapter, Index 13 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)