FastQCFastQC Report
Sat 31 Dec 2022
SRR1954578.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954578.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407551
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC41321.013860841955976No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41031.0067451680893924No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG34940.8573160168911376No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC26410.6480170579878346No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC18810.4615373290704721No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT14820.3636354713888569Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC13260.32535805334792456No Hit
GAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT10600.2600901482268477No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT10200.25027542565224964No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC8710.213715584061872No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCCATGGT8480.20807211858147814No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC7440.18255383988752327No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.17813721472895416No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTA6630.16267902667396228No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG6170.15139209571317452No Hit
ACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC5620.13789685217310227No Hit
GAATATGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC5480.13446169927199295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5040.12366550443993513No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCC4830.11851277508827117No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT4640.11385078186533709No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC4510.11066099702859275No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG4440.10894342057803807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)