FastQCFastQC Report
Sat 31 Dec 2022
SRR1954577.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954577.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159196
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10980.689715822005578No Hit
GAATCTATCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC9640.6055428528354984No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG7750.48682127691650545No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC5960.3743812658609513No Hit
GCTGTCACTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC4020.2525189075102389No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.20666348400713588No Hit
CTGTCTATTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT2930.18404985049875625Illumina Single End Adapter 2 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC2800.17588381617628582No Hit
CCTGTCACTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC2710.17023040779919094No Hit
GAACTGACTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT2520.15829543455865724No Hit
GAATGAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC2290.14384783537274806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.14384783537274806No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG2270.14259152240006032No Hit
GAATGAATGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT2040.1281439232141511No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC1650.10364582024673986No Hit
TCTGTCACTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC1620.10176135078770823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)