FastQCFastQC Report
Sat 31 Dec 2022
SRR1954576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206831
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52392.5329858676890793No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC33521.6206468082637517No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG27471.3281374648867916No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC22771.100898801436922No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT9950.481069085388554No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.4457745695761274No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTC8570.4143479459075283No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT8220.39742591777828273No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8010.3872727009007354No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGAAGGT7610.36793324018159757No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC7600.3674497536636191Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT6830.3302212917792787Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC5980.2891249377511108Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTA5880.2842900725713264No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC5220.2523799623847489No Hit
GAATATGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC5060.24464417809709377No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG4950.23932582639933087No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT3770.18227441727787422No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC3670.17743955209808976No Hit
GCCGATGGGACCAATAAACCTTTTGCTTCAGGGTATACTCTTGGGTTTTTT3210.1551991722710812No Hit
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC3110.15036430709129675Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC2520.12183860253056843Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGT2490.1203881429766331No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2420.11700373735078398No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC2280.11023492609908572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)