FastQCFastQC Report
Sat 31 Dec 2022
SRR1954575.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954575.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75575
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13731.816738339397949No Hit
GAATCTATCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC9471.2530598742970558No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG6440.8521336420774066No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC5700.7542176645716177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3880.5133972874627853No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.49884220972543825No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC2700.3572609990076083No Hit
GAACTGACTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT2410.31888852133642076No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG2200.2911015547469401No Hit
GAATGAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTC2120.2805160436652332No Hit
GAATGAATGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT1800.23817399933840555No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC1780.23552762156797882No Hit
GCTGTCACTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC1640.21700297717499173Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTATTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT1500.19847833278200464Illumina Single End Adapter 1 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.16539861065167052No Hit
CCTGTCACTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC1240.16407542176645717Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.15745947734039034No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.1481971551438968No Hit
GCCGATAGGACCAATAAACCTTTTGCTTCAGGGTATACTCTTGGGTTTTTT910.12041018855441614No Hit
GAATGCAGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT890.11776381078398941No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT850.11247105524313597No Hit
GAATGAAACGGCGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTA840.1111478663579226No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)