FastQCFastQC Report
Sat 31 Dec 2022
SRR1954573.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954573.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702061
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56230.8009275547281505No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG22970.3271795470763937No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC19050.2713439430476839No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC16340.23274330862987688No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC14780.21052301723069647No Hit
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC8430.12007503621480184Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC8160.11622921654955909Illumina PCR Primer Index 10 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)