FastQCFastQC Report
Sat 31 Dec 2022
SRR1954548.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954548.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604257
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123592.045321775337315No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG45000.7447162382893372No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC32270.5340442891021535No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC27850.46089660525240084No Hit
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC11380.18833046203850348Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCT8740.14464044272552906No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTT8420.13934468280880485No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTC6690.1107144807590148No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCT6640.10988701827202663No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGCAGAT6230.10310182587872378No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)