FastQCFastQC Report
Sat 31 Dec 2022
SRR1954547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435802
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72711.6684182266258532No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCG21940.503439635430769No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGC15640.35887857329704775No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTC13630.31275671061628907No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7970.18288121669932675No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC5830.13377634797453888TruSeq Adapter, Index 22 (95% over 22bp)
GGGCTTAACTTTCTCATGAATTTGCCTGAGGTTTCCAAACCCTTCACATTT4880.11197745765278727No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)