FastQCFastQC Report
Sat 31 Dec 2022
SRR1954544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566755
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78401.3833137775582043No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG26110.46069289199036617No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC18780.33136011151202904No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC15600.27525121084066306No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC7080.1249217033815317TruSeq Adapter, Index 21 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCT6180.1090418258330319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)