FastQCFastQC Report
Sat 31 Dec 2022
SRR1954533.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954533.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752743
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141121.8747434383315422No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG53140.7059514336234279No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC36430.4839633181577245No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC31730.42152500919968705No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21270.2825665599015866No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC16730.22225381039743977TruSeq Adapter, Index 22 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT9380.12461092298433861No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC8980.11929702434961202TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC8710.11571014277117156No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT8370.11119332893165396Illumina Paired End PCR Primer 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)