FastQCFastQC Report
Sat 31 Dec 2022
SRR1954527.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954527.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442946
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96052.1684358815747293No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG13280.2998108121531744No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10880.24562813525802243No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC10540.2379522560312092No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC7590.17135271568091823TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC7580.1711269545271884No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC6950.156904001842211TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGCT5260.11875036686187482Illumina Single End Adapter 1 (95% over 21bp)
AGCCTTGGGACAGTTGTTTCACTTCAACTGTGATTGTTTTTAACATTTTTT4550.10272132494705902No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)