FastQCFastQC Report
Sat 31 Dec 2022
SRR1954525.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954525.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133797
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16781.2541387325575313No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG6570.49104240005381283No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC5080.37967966396854935No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC4390.3281090009491992No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3020.22571507582382264No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC1570.11734194339185482Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT1370.1023939251253765No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)