FastQCFastQC Report
Sat 31 Dec 2022
SRR1954523.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954523.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746823
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94651.2673685732763988No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG35680.4777571124617212No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC25700.3441243775298833No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC22480.30100840493664494RNA PCR Primer, Index 16 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC20960.2806555234640604No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC16120.21584766403819913Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC12580.16844687429283778RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT10890.1458176837081879RNA PCR Primer, Index 16 (96% over 26bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCCCCCCTCGAATGC10220.13684634779593022No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8260.11060184273917649No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)