FastQCFastQC Report
Sat 31 Dec 2022
SRR1954521.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954521.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190763
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34411.8038089147266505No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCG10810.5666717340364746No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGC6510.3412611460293663No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC6370.3339221966523907No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC6050.31714745521930354No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT5760.30194534579556837No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC5600.29355797507902476No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5550.2909369217301049No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC2980.15621477959562388No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2940.154117936916488No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC2350.12318950739923361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)