FastQCFastQC Report
Sat 31 Dec 2022
SRR1954510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88972
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10121.1374364968754216No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG2400.26974778582025805No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1910.21467427954862203No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC1480.16634446792249247TruSeq Adapter, Index 16 (100% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGC1430.16072472238457045No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC1160.1303780964797914TruSeq Adapter, Index 16 (100% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC1120.12588230004945378No Hit
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT930.10452726700534999TruSeq Adapter, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)