FastQCFastQC Report
Sat 31 Dec 2022
SRR1954506.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954506.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1271415
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199221.56691560190811No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG88350.6948950578685952No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC69630.5476575311758946No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC55730.4383305215055667TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCCCCGCTCGAATGC36570.28763228371538796No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27330.2149573506683498No Hit
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC21520.1692602336766516TruSeq Adapter, Index 22 (95% over 24bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGCTCGAATGC16380.12883283585611308No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT14650.11522594904102908No Hit
GAACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT13840.10885509452067184No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTC13700.10775395917147429No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT13040.10256289252525728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)