FastQCFastQC Report
Sat 31 Dec 2022
SRR1954505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709389
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68770.9694258016405668No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21940.3092802397556207No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG13760.19396974015667004No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGC8810.12419138159740284No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC8300.11700209617008439Illumina PCR Primer Index 5 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC8030.11319600388503347No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)