FastQCFastQC Report
Sat 31 Dec 2022
SRR1954497.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954497.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292571
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCG38401.3125019226102381No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27790.949854907014024No Hit
GAATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC27190.9293470644732391TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGC24710.8445813153046612No Hit
GAACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCT8810.30112348797385935TruSeq Adapter, Index 13 (100% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTC6780.2317386207108702No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCT6540.2235354836945562No Hit
GCTGTCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTCTGC6110.20883819654032698TruSeq Adapter, Index 13 (96% over 25bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5960.2037112359051307No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCAACTCTCGTA5620.19209012513201923No Hit
GAATCTATCTCTTATACACATCTGACGCACCAACTCTCGTATGCCGTCTTC2990.10219741532824511TruSeq Adapter, Index 13 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)