FastQCFastQC Report
Sat 31 Dec 2022
SRR1954495.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954495.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173863
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17961.0329972449572364No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG12040.6924992666639825No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC7140.41066816976585013No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC6680.3842105565876581No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4370.2513473251928242No Hit
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC2160.12423574883672778TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTC2100.12078475581348534No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCT1790.1029546251933994No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)