FastQCFastQC Report
Sat 31 Dec 2022
SRR1954487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362462
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50341.3888352434186204No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCG16150.445563948772561No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTC11480.3167228564649536No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC9330.2574062936252628No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGC9040.24940545491665334No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCCTATCGTATGCCGTCTTCTGC4330.1194607986492377TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)