FastQCFastQC Report
Sat 31 Dec 2022
SRR1954485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303680
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36191.1917149631190727No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG21950.7228003161222339No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC17520.576923076923077No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC12970.4270943097997893No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6170.20317439409905164No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3970.13072971548998946No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCT3600.11854583772391993No Hit
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC3510.11558219178082192TruSeq Adapter, Index 21 (95% over 22bp)
ATGCAAGGATTGTAATTTTGAAATTTTGTTTAACAAAACTTCCATGTTTCA3320.10932560590094836No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTC3300.10866701791359325No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTA3120.10273972602739725No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)