FastQCFastQC Report
Sat 31 Dec 2022
SRR1954475.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1954475.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251581
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29311.1650323355102334No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7330.2913574554517233No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG3950.15700708718066947No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3070.12202829307459626No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC3020.12004086159129665RNA PCR Primer, Index 11 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC2600.10334643713157989No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)